Coding

Part:BBa_K2000000:Design

Designed by: Qin YE   Group: iGEM16_JSNU-China   (2016-10-04)


hKLF4-FL


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 486
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 486
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1464
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 486
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 486
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 313


Design Notes

In order to help our project on KLF4's function in cancer cell,we need express full-length of KLF4 gene.


Source

Capra hircus Kruppel-like factor 4 (KLF4) mRNA, complete cds。GenBank: KU041754.1； https://www.ncbi.nlm.nih.gov/nucleotide/1021691550?report=genbank&log$=nuclalign&blast_rank=10&RID=Z74WM35U015

References